Biosynthesis of 5-aminolevulinic Acid via the Shemin Pathway in a Green Sugar Beet Callus |
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Authors: | B. Bisbis J.P. Billard C. Huault C. Kevers F. Le Dily T. Gaspar |
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Affiliation: | (1) Hormonologie Fondamentale et Appliquée, Institut de Botanique B 22, Sart Tilman, B - 4000 Liège, Belgium;(2) Laboratoire de Physiologie et Biochimie Végétales (UA INRA 950), Institut de Recherche en Biologie Appliquée, Université de Caen, F - 14032 Caen Cedex, France |
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Abstract: | 5-Aminolevulinic acid synthase (ALAS) has been detected in a normal (auxin- and cytokinin-dependent) green sugar beet callus under light and under darkness. ALAS activity was lower when the callus was grown under light. The supply of precursors of the Shemin pathway (glycine and succinate) to dark-grown callus enhanced considerably the capacity of the 5-aminolevulinic acid (ALA) formation. Glutamate, -aminobutyrate or -ketoglutarate also increased ALA accumulation. Such an accumulation was also obtained after inhibition of polyamine synthesis. The results show that glutamate or its derivatives might feed the Shemin pathway in conditions preventing glutamate to be used through the Beale pathway. |
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Keywords: | 5-aminolevulinic acid synthase Beta vulgaris heterotrophy |
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