The gene encoding the NdhH subunit of type 1 NAD(P)H dehydrogenase is essential to survival of synechocystis PCC6803 |
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Authors: | Pieulle L Guedeney G Cassier-Chauvat C Jeanjean R Chauvat F Peltier G |
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Affiliation: | CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecophysiologie de la Photosynthèse, Saint-Paul-lez-Durance, France. |
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Abstract: | The physiological function of the type 1 NAD(P)H dehydrogenase (Ndh-1) of Synechocystis sp. PCC6803 has been investigated by inactivating the gene ndhH encoding a subunit of the complex. Molecular analysis of independent transformants revealed that all clones were heteroploid, containing both wild-type and mutant ndhH copies, whatever the metabolic conditions used during genome segregation, including high CO(2) concentration. By replacing the chromosomal copy of the ndhH gene by a plasmidial copy under the control of a temperature-controlled promoter, we induce a conditional phenotype, growth being only possible at high temperature. This clearly shows for the first time that an ndh gene is indispensable to the survival of Synechocystis sp. PCC6803. |
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