Novel immunochemical characteristics of glutathione S-transferase isozymes of rat liver cytosol |
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| Authors: | F Fukai M Kurano S Nishizawa Y Kiire T Nagai T Katayama |
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| Institution: | Department of Patho-Physiology, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan. |
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| Abstract: | Reevaluation of the immunochemical relationships among the individual glutathione S-transferase (GST) isozymes (GSTs 1-1, 1-2, 2-2, 3-3, 3-4, 4-4, and GSTs with isoelectric points of 7.5 and 6.8) of rat liver cytosol was performed utilizing the immunoblot technique. As a result, we found that the respective isozymes of two isozyme classes of rat liver cytosol might possess a common epitope(s) which has been undetected by the Ouchterlony double-diffusion method. The assumption was further supported by the results of the effects of Fab' prepared from some anti-GST antibodies on the enzymatic activity of GSTs. |
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