Medium scale production of L-myo-inositol 1-phosphate |
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| Authors: | F Feth R Wagner H Baydoun V Wray K G Wagner |
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| Institution: | Gesellschaft für Biotechnologische Forschung, Ag. Enzymologie, Braunschweig, Federal Republic of Germany. |
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| Abstract: | L-myo-Inositol-1-phosphate synthetase was purified from baker's yeast, grown in a fermenter in an inositol-deficient medium and analyzed using a new HPLC assay for inositol. This enzyme was used in a procedure, developed from methods partially described in the literature, for the medium scale production and purification of L-myo-inositol 1-phosphate. The identity and purity of the product were confirmed by 1H and 31P NMR spectroscopy. |
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