脐血造血细胞的体外扩增：1.生长规律的研究

考察了在添加细胞因子和未添加细胞因子培养条件下的造血细胞群体的生长和代谢，研究了长期培养条件下造血祖细胞生长规律。在静态培养条件下，脐血造血细胞群体的比生长速率为0.34d^-1，倍增时间为2d。培养后期，造血细胞消耗了大部分葡萄糖，乳酸浓度可达40 mmol/L。在造血细胞长期培养条件下，CFU-GM产出最高峰在培养第2周与第3周之间。BFU-E产出最高峰在培养第1周。每天换50%培养液，造血细胞总数扩增了14倍，CFU-GM扩增了13倍，BFU-E扩增了5倍。

The ex vivo Expansion of Cord Blood Hematopoietic Cells: 1. Cell Growth Behavior

The population growth and metabolism of hematopoietic cells from cord blood were investigated without and with hematopoietic growth factors and the long-term growth kinetics of cells was studied. In static cultivation of hematopoietic cells, the population specific growth rate was 0.34 d, and the average double time 2 days. The greater part of glucose was consumed and 40 mmol/L lactate was formed during cell culture. For the long-term culture, the maximum output of CFU-GM appeared between the 2nd and 3rd weeks and that of BFU-E in the first week. With 50% medium exchange, the expansions of total cells, CFU-GM, and BFU-E came up to 14, 13, and 5 times, respectively.