| 萝卜-芥蓝杂种F_1代创制及杂种鉴定 |
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| 引用本文： | 魏丽华,李象松,李炫丽,汪艳杰,王冰,吴江生,龙鸿. 萝卜-芥蓝杂种F_1代创制及杂种鉴定[J]. 武汉植物学研究, 2010, 28(2). DOI: 10.3724/SP.J.1142.2010.20243 |
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| 作者姓名： | 魏丽华 李象松 李炫丽 汪艳杰 王冰 吴江生 龙鸿 |
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| 作者单位： | 1. 华中农业大学生命科学技术学院,武汉,430070
2. 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 基金项目： | 教育部留学回国人员基金 |
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| 摘 要： | 以萝卜为母本,以芥蓝为父本,采用人工去雄授粉的方法进行杂交,然后通过胚抢救(embryo rescue)得到F1代植株,并利用RAPD技术对杂种F1代幼苗进行了鉴定.RAPD鉴定结果表明:杂种表现出亲本的特异带或者亲本不具备的新谱带,有的还遗失了亲本的特异带或者共有带.
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| 关 键 词： | 远缘杂交 胚抢救 |
Obtaining F_1 Hybrid and Hybrid Identification between Raphanus sativus L. and Brassica alboglabra Bailey |
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| WEI Li-Hua,LI Xiang-Song,LI Xuan-Li,WANG Yan-Jie,WANG Bing,WU Jiang-Sheng,LONG Hong. Obtaining F_1 Hybrid and Hybrid Identification between Raphanus sativus L. and Brassica alboglabra Bailey[J]. Journal of Wuhan Botanical Research, 2010, 28(2). DOI: 10.3724/SP.J.1142.2010.20243 |
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| Authors: | WEI Li-Hua LI Xiang-Song LI Xuan-Li WANG Yan-Jie WANG Bing WU Jiang-Sheng LONG Hong |
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| Affiliation: | WEI Li-Hua1,LI Xiang-Song1,LI Xuan-Li1,WANG Yan-Jie1,WANG Bing1,WU Jiang-Sheng2,LONG Hong1(1.College of Life Science & Technology,Huazhong Agricultural University,Wuhan 430070,China,2.National Key Laboratory of Crop Genetic Improvement,China) |
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| Abstract: | Using hand cross and embryo rescue,F1 plants from intergeneric Raphanus sativus L.(2n=18,RR) and Brassica alboglabra Bailey (2n=18,CC) hybrids were obtained.Random amplified polymorphic DNA (RAPD) markers were used to identify the F1 plants.The RAPD results indicated that the hybrids produced specific bands of parents or new bands absent in both parents.The F1 plants also lost some specific bands or common bands of both parents. |
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| Keywords: | RAPD |
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