Protein profile of symbiotic bacteria Mesorhizobium loti MAFF303099 in mid-growth phase |
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| Authors: | Kajiwara Hideyuki Kaneko Takayo Ishizaka Masami Tajima Shigeyuki Kouchi Hiroshi |
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| Institution: | Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. kajiwara@affrc.go.jp |
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| Abstract: | Expressed proteins in cultured symbiotic bacteria (Mesorhizobium loti MAFF303099) in the mid-growth phase were proteomically analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and capillary high-performance liquid chromatography coupled with an ion-trap mass spectrometry (MS). The genome sequence data of M. loti were used to identify the analyzed proteins. We identified 114 of the 127 proteins analyzed on 2D-PAGE gel with some microheterogenities which were caused by post-translational modifications. |
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