In vitro plant regeneration from embryogenic cultures of a diploid and a triploid,Cavendish banana |
| |
Authors: | Navarro Cuauhtémoc Escobedo Rosa Ma Mayo Alberto |
| |
Institution: | (1) Departamento de Biotecnología, Centro de Investigación Científica de Yucatán, A.C., Apartado 87 CORDEMEX, 97310 Mérida, Yucatán, México;(2) Present address: Calle Cuauhtémoc 72, Col. Del Carmen Coyoacán, 04100 México, D.F., México |
| |
Abstract: | Plant regeneration by somatic embryogenesis was attempted with diploid (Musa acuminata ssp. malaccensis) and triploid ('Grand
Nain') bananas. Explants inoculated in vitro were, respectively, immature zygotic embryos and male flower bud primordia. An
histological study showed that the embryogenic process involves a sequence of similar events for both species. A yellow-green
compact callus was initiated, which consisted of an actively dividing meristematic zone surrounded by several layers of starchy
cells. A white and friable callus, characterized by the presence of proembryonic cells, bicellular proembryos and proembryonal
masses in its periphery gradually appeared, which finally gave rise to somatic embryos from which plants were recovered. Induction
media contained 2,4-D (and also NAA and IAA for the triploid); zeatin and kinetin were necessary for embryo maturation and
6-BA and IAA were used for germination.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | histology Musa acuminata somatic embryogenesis regeneration |
本文献已被 SpringerLink 等数据库收录! |
|