CpG对乙型肝炎基因重组(CHO细胞)疫苗免疫效果的影响

为了研究CpG-寡脱氧核苷酸(CpG-OPN)作为佐剂对乙型肝炎基因重组(CHO细胞)疫苗(简称乙肝疫苗)免疫效果的影响,以乙肝疫苗加Al(OH)3、疫苗加CpG和疫苗加Al(OH)3与CpG3三种配伍方式,通过腹腔、皮下或肌内3种不同途径免疫Balb/c小鼠,观察不同免疫途径和不同配伍的免疫效果.同时又将疫苗与CpG混合后在4℃存放6个月再免疫小鼠,观察CpG的稳定性.结果表明:①3种免疫途径中以肌内注射效果最好,这在使用CpG的实验组尤为明显,在该组肌内免疫的ED50比腹腔的低了10倍,而诱发的抗体滴度提高了3倍;②疫苗与CpG、Al(OH)3联合使用的免疫效果最好,在肌内免疫时联合使用的免疫效果比疫苗+Al(OH)3提高4倍,比疫苗+CpG提高7倍;③疫苗+Al(OH)3免疫时,表现为IgG1抗体亚型占优势,而再加入CpG后则IgG1和IgG2a均升高,以IgG2a最显著;④疫苗与CpG混合后4℃保存半年,不影响其活性.

Effects of CpG on the Immune Responses to Recombinant Hepatitis B Virus Vaccine

In this paper,we studied the effects of CpG as adjuvant on the immune responses to recombinant hepatitis B virus vaccine.Firstly,the results showed that different immunization routes of CpG had great influence on the adjuvant activity. The ED 50 of vaccine with CpG inoculated by intramuscular route(I.M) was about 10 times lower than that by intraperitoneal route(I.P.),and the antibody titer was also about 3 times higher.These indicated that CpG had much better adjuvant activity by I.M.route than by I.P.route.Secondly,the study indicated that CpG could obviously release the inhibition of alum to Th1 immune response and induced strong Th1 response.Thirdly,when mice were immunized with vaccine containing alum as adjuvant,IgGl antibody response was predominant.When CpG was additionally added to the vaccine,both IgG1 and IgG2a were increased,especially the IgG2a.The data also showed that the combined use of CpG and alum produced significant synergetic adjuvant effect.Fourthly,the study proved that CpG could keep the immunostimulatory activities at least 6 months after mixed with the vaccine.