| 不同剂量x射线对大鼠精子CRISP2mRNA表达水平的影响 |
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| 引用本文： | 李仲辉,许羽龙,左超,邹练,江其生,李峰生,王思念,李晓. 不同剂量x射线对大鼠精子CRISP2mRNA表达水平的影响[J]. 现代生物医学进展, 2013, 0(23): 4424-4428 |
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| 作者姓名： | 李仲辉 许羽龙 左超 邹练 江其生 李峰生 王思念 李晓 |
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| 作者单位： | [1]山西医科大学研究生学院,山西太原030001 [2]解放军第二炮兵总医院泌尿外科,北京100088 [3]解放军第二炮兵总医院中心实验室,北京100088 |
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| 基金项目： | 全军医药卫生科研基金项目（06G016） |
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| 摘 要： | 目的：观察不同剂量x射线对大鼠精子CRISP2mRNA表达水平的影响，探讨其在电离辐射所致大鼠精子功能改变中的作用。方法：用吸收剂量为1、2、4、和6Gy的x射线分别照射活体SD大鼠的外生殖系统1…4812、24h后，用PCR技术检测精子CRISP2基因mRNA表达水平；用光学显微镜观察精予活力。以未照射组为对照。结果：4、6GyX射线照射不同时间（1、4、8、12、24h时）后大鼠精子的CRISP2mRNA相对表达量均较对照组显著下降（P．〈0．05），其中6Gb，照射24小时后相对表达量最低（P〈0．01），而4Gy照射组与6Gy照射组相比较差异无统计学意义（P〉0．05）；2Gyx射线照射8h后CRISP2mRNA相对表达量下降有统计学意义（P〈0．05）；2GyX射线照射1、4h后及1GyX射线照射不同时间（1、4、8、12、24la）后大鼠精子的CRISP2mRNA相对表达量较对照组下降，但差异无统计学意义（P〉O．05）。1、2GyX射线照射不同时间（1、4、8、12、24小时）及4GyX射线照射（1、4、8h）后，精子活力与正常对照组相比无明显改变（P〉0．05）；4GyX射线照射12、24h后大鼠精子活力显著低于正常对照；6GyX射线照射不同时间（1、4、8、12、24h）后，精子活力明显低于对照组（P〈0．05）。结论：不同剂量X射线照射不同时间可导致SD大鼠精子活力下降，这可能与其下调CRISP2基因的mRNA表达水平有关。
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| 关 键 词： | x射线 精子 CRISP2 mRNA |
Effects of X-ray Irradiation on the Expression Level of CRISP2 mRNA in Spermatogenesis of Male SD Rats |
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| LI Zhong-hui,XU Yu-long,ZUO Chao,ZOU Lian,JIANG Oi-sheng,LI Feng-sheng,WANG Si-nian,LI Xiao. Effects of X-ray Irradiation on the Expression Level of CRISP2 mRNA in Spermatogenesis of Male SD Rats[J]. Progress in Modern Biomedicine, 2013, 0(23): 4424-4428 |
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| Authors: | LI Zhong-hui XU Yu-long ZUO Chao ZOU Lian JIANG Oi-sheng LI Feng-sheng WANG Si-nian LI Xiao |
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| Affiliation: | 1 Graduate School of Shanxi Medical UniversiO＋, Taiyuan, Shanxi, 030001, China; 2 Department of Urology, General Hospital of the Second Am＇llery ofPLA, Beijing, 100088, China; 3 Center Laboratory, General Hospital of the Second Artillery ofPLA, Beijing, 100088, China) |
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| Abstract: | Objective： To observe the impact of expression levels of sperm CRISP2 mRNA with different dose and time-effects of x-ray irradiation on the rats. To explore the changes of sperm function of rats in ionizing radiation. Methods： The external reproductive system of SD rats were exposed to x-rays with the dose of 1,2,4 and 6 Gy respectively. The untreated rats were defined as the control group. The relative levels of CRISP2 mRNA expression by PCR and the motility （a ＋ b %） by optical microscope observation at 1,4,8,12 and 24 h after irradiation in sperm. Results： The levels of rats＇ sperm CRISP2 mRNA exposed by 4 or 6 Gy x-rays group was significantly lower than that of the control group （P〈0.01）; When accepted 2 Gy by irradiation, the levels of rats＇ CRISP2 mRNA have reduced to the different degree, but significant changes occured in exposure after 8 h （P〈0.05）; There was no change of CRISP2 mRNA with lower dose （1Gy）（P〉0.05）. There was no significantly decrease in 1,2 Gy and 4 Gy （P1,4,8）exposure sperm motility group （P〉0.05）; 4 Gy（12,24h）6 Gy exposure group sperm motility is obviously lower than that of the control group （P〈0.05）. Conclusion： Under certain condi- tions, dif- ferent dose X-ray irradiation at differ- ent times can lead to SD rats decreased sperm motility, which may be associated with down CRISP2 gene mRNA expression levels. |
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| Keywords: | X ray Sperm CRISP 2 mRNA |
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