微生物法检测实验动物血清中的抗生素残留

目的建立实验动物血清中抗生素残留检测方法,保证微生物质量检测结果的准确。方法采用微生物抑制法,用嗜热脂肪芽孢杆菌作为指示菌,对1640份实验动物血清进行抗生素残留检测。并与Premi@Test抗生素检测试剂盒进行比对。结果在受试样品中,检测出小鼠、大鼠、豚鼠、兔和犬的血清中存在抗生素残留,阳性率分别为27.2%、16.4%、39.9%、23.7%和47.6%。抽取82份血清和18份阴阳对照品,与Premi@Test检测的结果相比较差异不显著（X2=16.680,P〉0.05）。血清中抗生素的残留与DHL琼脂平皿的菌落生长有一定相关性（X2=9.939,P〈0.05）。结论本研究采用嗜热脂肪芽孢杆菌作为指示菌的微生物抑制法,能够对动物血清中的抗生素进行检测,具有良好的敏感性和重复性,成本低,操作简便。为实验动物体内抗生素残留检测和微生物学检测结果的准确性提供依据。

Antibiotic residue monitoring in laboratory animal serum by microbial inhibition test

Objective To develop a detection method for antibiotic residues in laboratory animal serum, and to ensure the accuracy of the results of the microbiological quality monitoring. Methods Antibiotic residues in 1640 serum samples of laboratory animals were tested by microbial inhibition assay of Bacillus stearothermophilgs, and the results were compared with that of the antibiotic detection kit Premi~ Test. Results Antibiotic residues were positive in sera of mice, rats, guinea pigs, rabbits and dogs, with a positive rate of 27. 2% , 16.4% , 39. 9% , 23.7% and 47. 6% , respectively. The results were consistent with that obtained with Premi~ test（X2 = 16. 680, P 〉 0. 05 ）. Serum antibiotic residues and DHL agar colony growth had certain correlation （X2 = 9. 939, P 〈 O. 05 ）. Conclusions The microbial inhibition method of Bacillus stearothermophilus used as an indicator is not only capable of detecting antibiotic residues in animal sera, low cost, and simple operation, but also has a good sensitivity and repeatability. This study provides a basis for detection of antibiotic residues in the laboratory animals, and makes up for the blank in the quality testing of laboratory animals.