特殊染色及免疫组化双重染色在恒河猴糖尿病模型中的应用

目的在链脲佐菌素（STZ）诱导恒河猴糖尿病动物模型基础上，用特殊染色、免疫组化双染方法显示胰腺等组织中的特征性病变。方法健康恒河猴5只，小剂量（30mg／kg）多次静脉注射STZ，濒死状态时将动物安乐死。取胰腺、心脏、肾脏、脾脏、肝脏、眼球、脑等器官制成石蜡切片，用HE、PAS、Masson、天狼星红和甲苯胺蓝等方法进行特殊染色，用免疫组化双重染色法同时显示胰岛A、B细胞。结果模型动物的胰岛萎缩，数量减少。Masson染色见外分泌部间质内纤维增生。局灶性慢性肾炎，病变区肾间质纤维组织增生，部分肾小球及肾小管萎缩。天狼星红染色见脾脏中央动脉管壁增厚。免疫组化双染见胰岛A胰高血糖素表达增多，胞浆呈棕褐色。B胰岛素表达减少，胞浆粉红色。结论HE染色结合特殊染色和免疫组化双重染色可较好地对STZ诱导糖尿病动物模型进行组织学评价。

Application of special and double immunohistochemical staining in experimental macaque diabetes models

Objective To use special and double immunohistochemical staining and assess the typical histopathologieal changes in experimental macaque diabetes models. Methods Streptozotoein was applied （30 mg/kg body weight, i. v. , for 3 days） to five macaques. The animals were sacrificed when impending death. Histopathological changes of the pancreas, heart, kidney and spleen were observed with HE, PAS, Masson, Sirius red, toluidine blue and double immunohistoehemical staining. Results The quantity of pancreatic islets was decreased obviously, and the reminders showed atrophy. Interstitial fibrotic hyperplasia was observed in the exoerine pancreas with Masson staining. There were focal ehronie nephritis, renal interstitial fibrotic hyperplasia, and partial glomerular and renal tubular atrophy. Thickened small arteries in the spleen were dipicted with PAS staining. By double immunohistochemistry staining, the quantity ofbrown-colored pancreatic islet A cells was increased while the amount of pink-colored B cells was decreased. Conclusions The use Of HE staining combined with special and double immunohistochemical staining is a better method to evaluate the histopathological changes in macaque models of diabetes mellitus.