肠易激综合征模型WHBE兔神经-体液-内分泌系统调节的变化

目的观察肠易激综合征（IBS）模型WHBE兔神经内分泌激素和电解质水平的变化，探讨神经-体液-内分泌系统在IBS中的作用。方法以湿热环境应激加小剂量致泻剂诱导WHBE兔建立IBS模型，在造模9d和14d时取血分离血清，检测血清中5-羟色胺（5-HT）、多巴胺（DA）、促肾上腺皮质激素（ACTH）、皮质醇（COR）、β-内啡肽（β-EP）激素和K^＋、Na^＋、cl^-含量的变化，并以JW兔作对照。结果与正常对照组比，造模9d时WHBE兔的5-HT、DA、ACTH、β-EP、COR均显著升高（P〈0．05，P〈0．01），Na^＋、K^＋含量均显著降低（P〈0．01），而JW兔ACTH、β-EP、COR均显著升高（P〈0．05，P〈0．01），K^＋含量显著降低（P〈0．05）；造模14d时WHBE兔5-HT、ACTH、β-EP均显著升高（P〈0．05，P〈0．01），JW兔ACTH显著升高（P〈0．05）；相关分析显示，IBS模型中血清中5-HT、COR与Na^＋、K^＋浓度呈显著负相关（P〈0．05，P〈0．01），血清中DA、β-EP水平与Na^＋、K^＋、Cl^-浓度呈显著负相关（P〈0．05，P〈0．01），ACTH水平则与K^＋浓度呈显著负相关（P〈0．01）。结论IBS的发生不仅与HPA轴兴奋性升高有关，亦与体液因素有关，故神经-体液-内分泌系统参与了WHBE兔IBS的发生过程。

Changes in the regulation of neurohumoral-endocrine system in the WHBE rabbit with irritable bowel syndrome

Objective To observe the changes of neuroendocrine hormone and electrolyte levels in WHBE rabbit with irritable bowel syndrome, and to explore the regulatory role of neurohumoral-endocrine system. Methods The rabbits model of irritable bowel syndrome was induced by moist heat, stress and stimulation with low-dose cathartic. Blood samples were taken at 9 d and 14 d of modeling, and the changes of serum dopamine （DA）, 5-hydroxytryptamine （5- HT）, adrenocorticotropie hormone （ACTH）, cortisol （COR）, β-endorphin （β-EP） hormone and K^＋ , Na^＋ , C1^- content were determined and compared with those of the JW rabbits. Results Compared with the normal control group, the DA,5-HT, ACTH, β-EP and COR contents were significantly increased （P 〈 0. 05, P 〈 0. 01 ） , and the Na^＋ , K^＋ contents were significantly lower （P 〈 0. 01 ） in the WHBE rabbit models at 9 days of modeling; and the ACTH, β-EP and cortisnl levels were significantly increased （ P 〈 0.05, P 〈 0. 01 ） , and K ^＋ content was significantly decreased （ P 〈 0.05 ） in the JW rabbits at 9 days of modeling. At 14 days of modeling, compared with the control group, the serum 5-HT, ACTH, β-EP were significantly increased （P 〈 0. 05, P 〈 0. 01 ） in the WHBE rabbit group, and the serum ACTH was significantly increased （P 〈 0. 05 ） in the JW rabbit group. The correlation analysis showed that in the IBS models there was a significant negative correlation between the serum 5-HT, COR and Na^＋ , K＋ concentrations （ P 〈 0. 05, P 〈 0. 01 ）, between the serum DA, β-EP levels and Na^＋ , K^＋ , C1- concentrations （P 〈 0.05, P 〈 0. 01 ）, and between the ACTH levels and K^＋ concentration （ P 〈 0. 01 ）. Conclusion Irritable bowel syndrome is not only associated with elevated excitability of the hippothalamus-pituitary-adrenal axis, but also related with humoral factors. Therefore, neurohumoral- endocrine system is involved in the pathohenesis of irritable bowel syndrome in WHBE rabbits.