| 猴免疫缺陷病毒衣壳蛋白p27在大肠杆菌中的表达及纯化 |
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| 引用本文： | 李敏,马兰芝,白杰英,刘一,赵彦斌,王洪宝,丁璐静,武文卿,孙兆增,曾林. 猴免疫缺陷病毒衣壳蛋白p27在大肠杆菌中的表达及纯化[J]. 中国实验动物学杂志, 2013, 0(1): 1-4 |
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| 作者姓名： | 李敏 马兰芝 白杰英 刘一 赵彦斌 王洪宝 丁璐静 武文卿 孙兆增 曾林 |
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| 作者单位： | [1]军事医学科学院实验动物中心,北京100071 [2]军事医学科学院科技部,北京100850 |
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| 基金项目： | 十二五重大专项“高致病病原动物模型研究”（2012ZX10004502） |
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| 摘 要： | 目的获得用于SIV检测的衣壳蛋白p27重组抗原。方法利用生物信息学软件选择衣壳蛋白p27抗原表位集中的区域,合成SIV p27基因;将该基因与pMAL-p5x载体连接构建pMAL-p5x-p27重组质粒,并转化大肠杆菌BL21中,诱导表达;用Amylose Resin亲和层析柱对表达产物进行纯化。结果 SDS-PAGE分析显示,pMAL-p5x-p27重组质粒可在大肠杆菌中高效表达,表达产物分子量约为70×103;经纯化获得目的蛋白p27纯度可达90%。结论本研究利用原核表达系统成功表达了SIV p27蛋白,为SIV检测方法的建立奠定了基础。
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| 关 键 词： | SIV p27 表达 纯化 |
Expression and purification of SIV capsid p27 in E.coli |
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| LI Min,MA Lan-zhi,BAI Jie-ying,LIU Yi,ZHAO Yan-bin,WANG Hong-bao,DING Lu-jing,WU Wen-qing,SUN Zhao-zeng,ZENG Lin. Expression and purification of SIV capsid p27 in E.coli[J]. Chinese Journal of Laboratory Animal Science, 2013, 0(1): 1-4 |
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| Authors: | LI Min MA Lan-zhi BAI Jie-ying LIU Yi ZHAO Yan-bin WANG Hong-bao DING Lu-jing WU Wen-qing SUN Zhao-zeng ZENG Lin |
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| Affiliation: | 1(1.Laboratory Animal Center of the Academy of Military Medical Sciences,Beijing 100071,China; 2.Department of Science and Technology,Academy of Military Medical Sciences,Beijing 100850,China) |
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| Abstract: | Objective To obtain capsid p27 of SIV for detecting. Methods The p27 gene of SIV was synthesized and inserted into pMAL-p5x vector to construct pMAL-pSx-p27 recombinant plasmid. And then the recombinant plasmid was transformed into E. coli BL21 to express protein and the express product was purified by Amylose Resin maltose-binding protein affinity column. Results SDS-PAGE analysis indicated that pMAL-p5x-p27 can be expressed in E. coli BL21 and the protein molecular weight was about 70 x 103 ; The final p27 protein purification could reach 90%. Conclusion p27 of SIV was successfully expressed in E. coli, which lays a foudation for developing the methods of detecting SIV antibody. |
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| Keywords: | SIV p27 Prokaryotic expression Purification |
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