Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12. |
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Authors: | Y C Sung D Parsell P M Anderson and J A Fuchs |
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Abstract: | The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source. |
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