Mass spectrometric analysis of cytokinins in plant tissue VII. Quantification of cytokinin bases by negative ion mass spectrometry |
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| Authors: | D S Letham S Singh O C Wong |
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| Institution: | 1. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, GPO Box 475, 2601, Canberra, ACT, Australia
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| Abstract: | A study of derivatives of N6-(isopent-2-enyl)adenine formed by substitution at N-9 indicated that sensitivity of detection by chemical ionization mass spectrometry was maximized by a pentafluorobenzyl substituent and negative ion monitoring. O-t-Butyldimethylsilyl-9-pentafluorobenzyl derivatives of zeatin (Z),cis-zeatin (cis-Z), and dihydrozeatin (DZ) were characterized by mass spectrometry. A procedure was based on these stable derivatives and negative ion chemical ionization mass spectrometry for quantification of zeatin and dihydrozeatin in plant tissue. |
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