Indole-3-acetic acid and indole-3-butyric acid in tissues of carrot inoculated withAgrobacterium rhizogenes |
| |
Authors: | Ephraim Epstein Scott J Nissen Ellen G Sutter |
| |
Institution: | 1. Department of Horticulture, Volcani Center, Bet Dagan 2. Department of Agronomy, University of Nebraska, 68583, Lincoln, Nebraska, USA 3. Department of Pomology, University California, 95616, Davis, California, USA
|
| |
Abstract: | The role of auxins in induction of roots byAgrobacterium rhizogenes was studied in carrot root disks. Transformed roots were produced on root disks by inoculation withA. rhizogenes, A4. Measurement of indole-3-acetic acid (IAA) by gas chromatography-mass spectrometry (GC-MS) indicated that there was a significant increase in the concentration of IAA in transformed callus and induced roots compared with initial IAA concentrations in carrot disks. Indole-3-butyric acid (IBA) was found to occur naturally in carrot roots. The presence of IBA, a potent root inducer, must be taken into account when assessing the role of auxin during transformation and induction of roots byA. rhizogenes. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|