Structure and expression of the Lyt-3
agene of C.AKR mice |
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Authors: | Hyun J Youn June V Harriss Paul D Gottlieb |
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Institution: | (1) Department of Microbiology, University of Texas at Austin, 78712 Austin, TX, USA |
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Abstract: | The mouse Lyt-3
agene, which encodes the Lyt-3.1 T-cell surface alloantigen of the C.AKR strain, has been cloned, and the nucleotide sequence of its exons and more than 2 kb of 5 flanking sequence have been determined. The gene extends over approximately 16 kb of DNA and consists of six exons encoding leader, leader plus V-like domain, membrane-proximal, transmembrane, and cytoplasmic domains. The only difference between the coding region of the Lyt-3
agene and the cDNA sequences reported for Lyt-3
b(Nakauchi et al. 1987, Panaccio et al. 1987) is at position 77 of the mature protein where Lyt-3
aencodes serine and Lyt-3
bencodes arginine. This substitution must therefore be the basis for the serological distinction between the Lyt-3.1 and Lyt-3.2 alloantigens. Potential TATA and CAAT sequences, two Sp1 protein binding sites, two extended repeats of the dinucleotide, CA, a number of short inverted repeats, and an inverted segment of the mouse B1 repetitive sequence are found 5 to the Lyt-3
agene. Two consensus poly-A addition signals and a complete copy of the mouse B1 sequence are found 3 to the gene. Both B1-related regions are flanked by short direct repeats suggesting that they arose by an insertional mechanism. Cotransfection of the Lyt-3
agene together with a cloned Lyt-2
agene resulted in expression of both Lyt-2 and Lyt-3.1 on the surface of Ltk– and BW5147 cells. Transfection of the Lyt-3
agene without Lyt-2
aled to expression of Lyt-3-related cellular RNA but did not result in surface expression of Lyt-3.1, suggesting that the Lyt-3 glycoprotein is not expressed on the cell surface in the absence of Lyt-2. |
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