Vasopressin-induced changes in receptor-mediated endocytosis of asialoglycoprotein in rat hepatocytes. |
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Authors: | S Gil-Falgon C Lamaze J Feger |
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Affiliation: | Laboratoire de Biochimie, UFR des Sciences Pharmaceutiques et Biologiques, université Paris-Sud, Chatenay-Malabry, France. |
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Abstract: | The ability of second messengers to modulate receptor-mediated endocytosis was studied on isolated rat hepatocytes. A 20-min preincubation with vasopressin was used as a modulation. We observed a 20% inactivation of both surface and intracellular receptors, with no change in the affinity of those remaining active. The internalization and dissociation of a synchronous wave of ligand was not affected, but its degradation was partially inhibited. Our observations suggest that second messengers such as intracellular calcium and diacylglycerol play a complex role in the intracellular trafficking associated with endocytosis. |
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Keywords: | vasopressin endocytosis asialoglycoprotein-receptor hepatocytes |
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