Breakdown ofd-glucose by mixed cultures ofEscherichia coli,Desulfovibrio vulgaris,andChromatium vinosum |
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Authors: | P A Loka Bharati Renee Baulaigue Robert Matheron |
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Institution: | 1. Laboratoire de Microbiologie, Faculté des Sciences de St. Jér?me, Rue Henri Poincaré, F-13397, Marseille Cedex 4, France
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Abstract: | Experiments with mixed cultures ofEscherichia coli, Desulfovibrio vulgaris, andChromatium vinosum were conducted using a synthetic medium with glucose as the substrate. The bacterial number and the changes in the chemical
factors were determined during the development of the mixed culture. (i) Fermentation of glucose byE. coli produces organic acids (formic, acetic, lactic, and succinic) and alcohol. The growth-yield constant K (cell dry weight per
weight solid substrate) does not exceed 0.05. (ii) In the mixed culture ofE. coli andD. vulgaris, the reduction of sulfate is accompanied by a total consumption of formate, lactate, and alcohol and an increase in the sulfide
and acetate content. (iii) When the three physiologically different species are allowed to grow simultaneously, there is no
accumulation of catabolites in the medium and the growth yield constant increases to 0.46. Maximum phototrophic production
requires the presence of bothE. coli andD. vulgaris. A low substrate concentration and the simultaneous growth of the three organisms are other factors that contribute towards
a high output. The biochemical parameters of the medium are influenced to a large extent by the glucose level. The results
suggest that the behavior of the strains is different in pure and mixed cultures. |
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