Embedding Iliac Bone Biopsies at Low Temperature using Glycol and Methyl Methacrylates |
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| Authors: | Daniel Chappard Christian Alexandre Marcel Camps Jean Pierre Montheard Georges Riffat |
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| Institution: |
a Laboratory of Bone Biology, University of Saint Etienne, North Hospital-Rheumatology Division, 42277 Saint Priest en Jarez Cedex, and Laboratory of Macromolecular Chemistry, University of Saint Etienne, U. E. R. of Sciences, Saint Etienne, Cedex, France |
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| Abstract: | A mixture of pure and anhydrous glycol methacrylate and methyl methactylate is used as an embedding medium for iliac bone biopsies. Infiltration is carried out at -20 C with the embedding medium and a cold inactivated catalyst-initiator system. Raising the temperature to 4 C initiates polymerization and limits the peak temperature of polymerization to 25 C. In this way, such thermolabile enzymes as osteoclastic acid phosphatase are preserved. After staining, sections are dehydrated in polyethylene glycol 400 30% in 2-propanol. This gives flat sections and improves staining properties. |
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