The collagen-binding site of type-II units of bovine seminal fluid protein PDC-109 and fibronectin |
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Authors: | L Bányai M Trexler S Koncz M Gyenes G Sipos L Patthy |
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Institution: | Institute of Enzymology, Hungarian Academy of Sciences, Budapest. |
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Abstract: | A single type-II domain has been isolated by limited proteolysis of the collagen-binding bovine seminal fluid protein, PDC-109. The 45-residue fragment corresponding to the second type-II domain of the parent molecule was found to have retained affinity for immobilized collagen, indicating that this minidomain carries critical regions of the collagen-binding site. Studies on various fragments of fibronectin have also implicated the two type-II units of this molecule in collagen-binding. In the present work we have found that type-II domains of human fibronectin, expressed in Escherichia coli as beta-galactosidase fusion proteins, bind specifically to immobilized collagen. |
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