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Effect of ubiquinone extraction on ubiquinol-1 oxidase activity in beef heart mitochondria |
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| Authors: | P Pasquali L Landi L Cabrini G Lenaz |
| Institution: | (1) Istituto di Chimica Biologica, Facoltà di Farmacia, University of Bologna, Italy;(2) Istituto ed Orto Botanico, Facoltà di Scienze, University of Bologna, Italy;(3) Istituto di Chimica Biologica, Via Irnerio, 48, 40126 Bologna, Italy |
| Abstract: | Extraction of endogenous ubiquinone with different methods does not influence ubiquinol oxidase activity in lyophilized mitochondria in terms ofK M, although a decrease ofV max is sometimes observed. Experiments with submitochondrial particles from a UQ-deficient mutant ofS. cerevisiae confirm the results with UQ-depleted mitochondria and support the idea that endogenous ubiquinone is not required for the oxidation of exogenous ubiquinols by complex III. |
| Keywords: | Ubiquinone ubiquinols ubiquinone-depleted mitochondria ubiquinol oxidase ubiquinone-deficient mutant |
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