Separation and purification of useful proteins using hydrogel ultrafiltration |
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Authors: | Chang-Ho Park Chang Kyu Son Jong Hwa Park In Sik Chung |
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Institution: | (1) Department of Chemical Engineering, Kyung Hee University, Suwon, Korea;(2) Department of Genetic Engineering, Kyung Hee University, Suwon, Korea |
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Abstract: | The hydrogel process is a different form of ultrafiltration and has been used to separate biological molecules. In this study,
the gel pore size was predicted by pulse NMR technique and neural network using a data base obtained from gel filtration chromatography
and diffusion experiment. Recombinant alkaline phosphatase expressed in insect cells was concentrated 1.5 times by hydrogel
ultrafiltration by swelling at 20°C and collapsing at 35°C at 53–65% separation efficiency and 78–83% enzyme recovery. Wild
and recombinantAutographa californica nuclear polyhedrosis viruses (AcNPV) were also concentrated 1.4 and 1.6 times of the feed solution at 48.5 and 60.0% separation
efficiency, respectively. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of AcNPV
and recombinant proteins from insect cells. |
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Keywords: | NMR neural network Autographa californica nuclear polyhedrosis viruses insect cells recombinant alkaline phosphatase |
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