A multiprotein complex that mediates translational enhancement in Drosophila |
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Authors: | Nelson Meryl R Luo Hua Vari Heli K Cox Brian J Simmonds Andrew J Krause Henry M Lipshitz Howard D Smibert Craig A |
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Affiliation: | Department of Biochemistry, University of Toronto, 1 King's College Circle,Toronto, Ontario, Canada. |
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Abstract: | Modulating the efficiency of translation plays an important role in a wide variety of cellular processes and is often mediated by trans-acting factors that interact with cis-acting sequences within the mRNA. Here we show that a cis-acting element, the Hsp83 degradation element (HDE), within the 3'-untranslated region of the Drosophila Hsp83 mRNA functions as a translational enhancer. We show that this element is bound by a multiprotein complex, and we identify components using a novel affinity-based method called tandem RNA affinity purification tagging. Three proteins (DDP1, Hrp48, and poly(A)-binding protein) are components of the HDE-binding complex and function in translational enhancement. Our data support a model whereby the HDE is composed of several cis-acting subelements that represent binding sites for trans-acting factors, and the combined action of these trans-acting factors underlies the ability of the HDE to stimulate translation. |
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