Raw starch-degrading enzyme from newly isolated strains of endophytic fungi |
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| Authors: | Marlida Yetti Saari Nazamid Hassan Zaiton Radu Son |
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| Institution: | (1) Department of Animal Nutrition, Faculty of Animal Science, University Andalas, Kampus Limau Manis Padang, 25163 Unand Padang, Indonesia;(2) Department of Food Science, Faculty of Food Science and Biotechnology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;(3) Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia |
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| Abstract: | Four newly isolated strains of endophytic fungi namely Gibberella pulicaris, Acremonium sp., `Synnematous' sp. and Nodilusporium sp. were compared for their degradative activity on raw and gelatinized starches, substrate specificity and optimum pH. Results
showed that the raw starch-degrading enzyme from Acremonium sp. had a broad activity towards both small and large granule size of raw starches while the enzyme from other strains showed
high activity toward starches of smaller granule size. Analysis of the end product by TLC showed that enzyme from Gibberella pulicaris, Acremonium and Nodilusporium sp. hydrolysed raw sago starch to produce solely glucose but the enzyme of `Synnematous' sp. produced glucose and maltose.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Endophytic fungi raw starch-degrading ability raw starch-degrading enzyme |
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