Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies |
| |
| Authors: | Elif Nurtop Paola Mariela Saba Villarroel Boris Pastorino Laetitia Ninove Jan-Felix Drexler Yelin Roca Bouba Gake Audrey Dubot-Peres Gilda Grard Christophe Peyrefitte Stéphane Priet Xavier de Lamballerie Pierre Gallian |
| |
| Affiliation: | 1.Unité des Virus émergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection),Marseille,France;2.Virología II,Centro Nacional de Enfermedades Tropicales (CENETROP),Santa Cruz de la Sierra,Bolivia;3.Institute of Virology,Charité-Universit?tsmedizin Berlin,Berlin,Germany;4.Centre Pasteur du Cameroun,Yaoundé,Cameroon;5.National Reference Centre for Arboviruses,French Armed Forces Biomedical Research Institute,Marseille,France;6.Laboratoire de Virologie,établissement Fran?ais du Sang Alpes Méditerranée (EFS),Marseille,France |
| |
| Abstract: | Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
