The anti-ageing molecule sirt1 mediates beneficial effects of cardiac rehabilitation |
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Authors: | Giusy Russomanno Graziamaria Corbi Valentina Manzo Nicola Ferrara Giuseppe Rengo Annibale A. Puca Salvatore Latte Albino Carrizzo Maria Consiglia Calabrese Ramaroson Andriantsitohaina Walter Filippelli Carmine Vecchione Amelia Filippelli Valeria Conti |
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Affiliation: | 1.Department of Medicine, Surgery and Dentistry,University of Salerno,Baronissi,Italy;2.Department of Medicine and Health Sciences,University of Molise,Campobasso,Italy;3.Department of Translational Medical Sciences,Federico II University of Naples,Naples,Italy;4.Salvatore Maugeri Foundation,IRCCS, Scientific Institute of Telese Terme,Benevento,Italy;5.IRCCS MultiMedica,Milan,Italy;6.Cardiac Rehabilitation Unit of “San Gennaro dei Poveri” Hospital,Naples,Italy;7.Vascular Physiopathology Unit, IRCCS INM Neuromed,Pozzilli,Italy;8.INSERM U1063, Stress Oxydant et Pathologies Métaboliques,Institut de Biologie en Santé, Université d’Angers,Angers,France;9.Department of Institutional Study and Territorial Systems,University of Naples “Parthenope”,Naples,Italy |
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Abstract: | BackgroundAn exercise-based Cardiac Rehabilitation Programme (CRP) is established as adjuvant therapy in heart failure (HF), nevertheless it is underutilized, especially in the elderly. While the functional and hemodynamic effects of CRP are well known, its underlying molecular mechanisms have not been fully clarified. The present study aims to evaluate the effects of a well-structured 4-week CRP in patients with stable HF from a molecular point of view.ResultsA prospective longitudinal observational study was conducted on patients consecutively admitted to cardiac rehabilitation. In fifty elderly HF patients with preserved ejection fraction (HFpEF), levels of sirtuin 1 (Sirt1) in peripheral blood mononuclear cells (PBMCs) and of its targets, the antioxidants catalase (Cat) and superoxide dismutase (SOD) in serum were measured before (Patients, P) and at the end of the CRP (Rehabilitated Patients, RP), showing a rise of their activities after rehabilitation.Endothelial cells (ECs) were conditioned with serum from P and RP, and oxidative stress was induced using hydrogen peroxide. An increase of Sirt1 and Cat activity was detected in RP-conditioned ECs in both the absence and presence of oxidative stress, together with a decrease of senescence, an effect not observed during Sirt1 and Cat inhibition.ConclusionsIn addition to the improvement in functional and hemodynamic parameters, a supervised exercise-based CRP increases Sirt1 activity and stimulates a systemic antioxidant defence in elderly HFpEF patients. Moreover, CRP produces antioxidant and anti-senescent effects in human endothelial cells mediated, at least in part, by Sirt1 and its target Cat. |
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