Determination (by methylation analysis) of the substitution pattern of 2-amino-2-deoxyhexitols obtained from O-glycosylic carbohydrate units of glycoproteins |
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Authors: | Jukka Finne Heikki Rauvala |
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Affiliation: | Department of Medical Chemistry, University of Helsinki, SF-00170 Helsinki 17 Finland |
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Abstract: | The derivatives obtained by per-methylation of unsubstituted 2-amino-2-deoxy-hexitols and of these compounds monosubstituted at C-3. C-4, or C-6, and disubstituted at C-3 and C-6, have been analysed by g.l.c.-m.s. Each derivative can be identified on the basis of retention time and mass spectrum. In methylation analysis, methanolysis gave one derivative of each hexitol, whereas a mixture of products was formed when degradation was effected by acetolysis followed by hydrolysis. An application in the analysis of amino-sugar linkages in alkali-labile O-glycosylic oligosaccharides from rat-brain glycoproteins is described. |
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