Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets |
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Authors: | J M Myers P W Simon |
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Institution: | (1) Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA, US;(2) USDA Vegetable Crops Research, Department of Horticulture,1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA Fax: (608) 262-4743 E-mail: psimon@facstaff.wisc.edu, US |
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Abstract: | Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production.
The best callus production occurred on root segments initially cultured on medium with 4,5 μm 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μm 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μm 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and
then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively)
occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
Received: 14 October 1997 / Revision received: 26 December 1997 / Accepted: 12 January 1998 |
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Keywords: | Garlic Allium sativum L Root segments Regeneration Picloram |
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