Distribution of ClC-2 chloride channel in rat and human epithelial tissues |
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Authors: | Lipecka Joanna Bali Moëz Thomas Annick Fanen Pascale Edelman Aleksander Fritsch Janine |
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Affiliation: | Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 467, Faculté de Médecine Necker-Enfants Malades, 75015 Paris, France. |
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Abstract: | The ubiquitous ClC-2 Cl channel is thought to contribute to epithelial Cl secretion, but the distribution of the ClC-2 protein in human epitheliahas not been investigated. We have studied the distribution of ClC-2 inadult human and rat intestine and airways by immunoblotting andconfocal microscopy. In the rat, ClC-2 was present in the lateralmembranes of villus enterocytes and was predominant at the basolateralmembranes of luminal colon enterocytes. The expression pattern of ClC-2in the human intestine differed significantly, because ClC-2 was mainlydetected in a supranuclear compartment of colon cells. We foundsignificant expression of ClC-2 at the apex of ciliated cells in bothrat and human airways. These results show that the distribution ofClC-2 in airways is consistent with participation of ClC-2 channels inCl secretion and indicate that extrapolation of resultsfrom studies of ClC-2 function in rat intestine to human intestine isnot straightforward. |
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