Acetylcholinesterase from Drosophila melanogaster. Identification of two subunits encoded by the same gene |
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Authors: | D Fournier J M Bride F Karch J B Bergé |
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Institution: | INRA, Centre de Recherche d'Antibes, France. |
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Abstract: | Purified acetylcholinesterase from Drosophila melanogaster is composed of a 55 kDa and a 16 kDa noncovalently associated subunit. Cleavage of disulfide bonds reveals that two 55 kDa polypeptides are linked together in native dimeric AChE. Western blots with two antibodies directed against the N- and C-termini of the predicted AChE primary sequence show that the 55 and 16 kDa polypeptides originate from proteolysis of the same precursor encoded by the Ace locus. |
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