Genetic analysis of the bchC and bchA genes of Rhodobacter sphaeroides |
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Authors: | Peter McGlynn and C. Neil Hunter |
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Affiliation: | (1) Krebs Institute for Biomolecular Research, the University of Sheffield, Western Bank, S10 2TN Sheffield, UK |
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Abstract: | Summary This study has identified by sequence analysis a single gene in the bchC locus of Rhodobacter sphaeroides and three genes, designated bchX, Y and Z, in the bchA locus, which was previously thought to contain only a single gene. All four genes may reside within the same operon and are transcribed in the order bchC-X-Y-Z. Complementation analysis of eight transposon insertion mutants within these genes suggests that bchX, Y and Z are essential for the reduction of 2-devinyl-2hydroxyethyl chlorophyllide a and that bchC encodes the 2-desacetyl-2-hydroxyethyl bacteriochlorophyllide a dehydrogenase. Similarity between the putative BchX protein and dinitrogenase reductase proteins suggests that BchX may also be a reductase, supplying electrons for reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a. |
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Keywords: | Rhodobacter sphaeroides Bacteriochlorophyll biosynthesis bchCA |
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