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The Anopheles albimanus white gene: molecular characterization of the gene and a spontaneous white gene mutation |
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| Authors: | Zhaoxi Ke Mark Q. Benedict Anthony J. Cornel Nora J. Besansky Frank H. Collins |
| Affiliation: | (1) Entomology Branch, Mailstop F22, Division of Parasitic Diseases, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA;(2) Department of Biology, Emory University, Atlanta, GA 30322, USA;(3) Department of Biological Sciences, Galvin Life Sciences, University of Notre Dame, Notre Dame, IN 46556, USA;(4) Mosquito Control Research Laboratories, Kearney Agricultural Experimental Field Station, University of California, 9240 South Riverbend Ave, Parlier, CA 93648, USA;(5) University of Notre Dame, USA |
| Abstract: | We have cloned and characterized the white gene of Anopheles albimanus. Comparison of the deduced amino acid sequence of this white gene with its homologs from six species of Diptera show that the An. albimanus gene is most similar to the white gene of An. gambiae (92% identity). A spontaneous white-eyed mutant An. albimanus was caused by an approximately 10 kb insertion into a CT dinucleotide repeat region of intron 2 of the white locus. The flanks of this insertion are long (at least 400 bp), nearly perfect inverted terminal repeat sequences. This cloned white gene should be useful as a marker for germ line transformation of An. albimanus. This revised version was published online in July 2006 with corrections to the Cover Date. |
| Keywords: | Anopheles albimanus malaria vector transformation white gene |
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