Multiple forms of acid phosphatase and their differential secretion during stalk formation under submerged monolayer incubation of Dictyostelium discoideum |
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Authors: | A A Oohata |
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Abstract: | The electrophoretic pattern of intracellular and secreted acid phosphatases (AcPases) in Dictyostelium discoideum was examined during incubation of the cells as a submerged monolayer. Three distinct forms of the enzyme were observed in the cell during differentiation; one was detected throughout development (AcPase 1), whereas the others including AcPase 2 were stage-specific. AcPase 1 was released in the medium predominantly in early development and AcPase 2, a prestalk specific form, was secreted during stalk formation. When cells were incubated under conditions where stalk cells did not form, only AcPase 1 was recognized both in the cell and in the medium. |
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