Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon |
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| Authors: | Aron M Geurts Andrew Wilber Corey M Carlson Paul D Lobitz Karl J Clark Perry B Hackett R Scott McIvor David A Largaespada |
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| Institution: | (1) Department of Genetics, Cell Biology, and Development and The Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, MN 55455, USA;(2) Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA;(3) University of Minnesota Cancer Center, Minneapolis, MN 55455, USA |
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| Abstract: | Background Insertional mutagenesis techniques with transposable elements have been popular among geneticists studying model organisms
from E. coli to Drosophila and, more recently, the mouse. One such element is the Sleeping Beauty (SB) transposon that has been shown in several studies to be an effective insertional mutagen in the mouse germline. SB transposon
vector studies have employed different functional elements and reporter molecules to disrupt and report the expression of
endogenous mouse genes. We sought to generate a transposon system that would be capable of reporting the expression pattern
of a mouse gene while allowing for conditional expression of a gene of interest in a tissue- or temporal-specific pattern. |
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