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Yeast Catalysed Reduction of β-Keto Esters (2): Optimisation of the Stereospecific Reduction by Zygosaccharomyces Rouxii |
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| Authors: | Keith O Hallinan David H G Crout Jonathan R Hunt Anthony S Carter Howard Dalton J Colin Murrell Robert A Holt John Crosby |
| Institution: | a Department of Chemistry, University of Warwick, Coventry, UK b Department of Biological Sciences, University of Warwick, Coventry, UK c ZENECA Bio Products, Billingham, Cleveland, UK d ZENECA Pharmaceuticals, Macclesfield, Cheshire, UK |
| Abstract: | Zygosaccharomyces rouxii catalysed the reduction of ethyl 4-chloroacetoacetate (ethyl 4-chloro-3-oxobutanoate) to the corresponding (S)-hydroxy ester (ethyl (S)-4-chloro-3-hydroxybutanoate) in high enantiomeric excess. The productivity of non-immobilised cells was compared to cells immobilised on a range of organic and inorganic supports. Cells immobilised in calcium alginate displayed a catalytic activity significantly higher than that of non-immobilised cells. A time dependent fall in the enantiomeric purity of the product was observed with the use of this matrix. This phenomenon was not seen in the reduction catalysed by non-immobilised cells. |
| Keywords: | Immobilisation alginate ethyl 4-chloro-3-hydroxybutanoate bioreduction Zygosaccharomyces rouxii |
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