| 渗透胁迫诱导的小麦叶片细胞程序性死亡 |
| |
| 引用本文： | 林久生,王根轩. 渗透胁迫诱导的小麦叶片细胞程序性死亡[J]. 植物生理与分子生物学学报, 2001, 27(3): 221-225 |
| |
| 作者姓名： | 林久生 王根轩 |
| |
| 作者单位： | 林久生（兰州大学干旱农业生态国家重点实验室，兰州730000） 王根轩（兰州大学干旱农业生态国家重点实验室，兰州730000） |
| |
| 基金项目： | 国家自然科学基金（39770447）和国家重点基础研究专项经费（G1999011705）资助。 |
| |
| 摘 要： | 用20%PEG6000（-0.63MPa）溶液对小麦（Triticumaestivum）根系进行渗透胁迫，在DNA琼脂糖凝胶电泳图谱上观察到明显的梯状DNA条带，表明PEG处理诱发了DNA核小体间的断裂，从而表现出典型的细胞程序性死亡的生化特征；末端脱氧核糖核酸转移酶介导的3′-OH末端标记法（terminaldeoxynucleotidyl transferase(TdT)-mediated dUTP nick end
|
| 关 键 词： | 渗透胁迫，小麦叶片，细胞程序性死亡，DNA梯状条带，TUNEL |
| 修稿时间： | 2000-09-30 |
Programmed Cell Death Induced by Osmotic Stress in Wheat Leaves |
| |
| LIN Jiu-Sheng WANG Gen-Xuan. Programmed Cell Death Induced by Osmotic Stress in Wheat Leaves[J]. Journal Of Plant Physiology and Molecular Biology, 2001, 27(3): 221-225 |
| |
| Authors: | LIN Jiu-Sheng WANG Gen-Xuan |
| |
| Abstract: | PEG solution (-0.63 MPa) was used as osmotic stress to induce cell death in wheat (Triticum aestivum) leaves. The DNA ladder, which is the typical character of programmed cell death (PCD), was observed in the leaves after being treated for 3 to 5 days (Fig.1). Under the same conditions, positive reaction to the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) procedure also appeared (Fig.2). These results indicated that osmotic stress could induce PCD in wheat leaves. During PCD, the decrease in chlorophyll and protein contents and the increase in electrolyte leakage were all slower than those during necrosis (Fig.3). |
| |
| Keywords: | osmotic stress wheat leaves programmed cell death DNA ladder TUNEL |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
