| 萝卜色素的分离、纯化及其性质研究 |
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| 引用本文： | 陈清轩,张志红. 萝卜色素的分离、纯化及其性质研究[J]. 中国生物化学与分子生物学报, 1993, 9(4): 424-428 |
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| 作者姓名： | 陈清轩 张志红 |
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| 作者单位： | 中国科学院发育生物学研究所,中国科学院发育生物学研究所 北京 100080,北京 100080 |
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| 摘 要： | “心里美”萝卜酒精提取物,经薄层层析、高压液相层析纯化后可得到天然色素为粉红色针状结晶。该色素性质稳定、耐温、耐光,颜色随pH值的不同而变化。经红外光谱分析和质谱鉴定,初步推导其结构可能为报春花色素。
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| 关 键 词： | 萝卜色素 高压液相层析 结构分析 |
| 收稿时间： | 1993-08-20 |
Isolation, Purification and Characterization of Radish Pigments |
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| Chin,Qing-xuan Zhang,Zhi-hong. Isolation, Purification and Characterization of Radish Pigments[J]. Chinese Journal of Biochemistry and Molecular Biology, 1993, 9(4): 424-428 |
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| Authors: | Chin Qing-xuan Zhang Zhi-hong |
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| Affiliation: | (Institute of Developmental Biology, Academia Sinica, Beijing 100080 |
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| Abstract: | Anthocyanin pigments in "Xinlimei" radish were isolated and purified by using alcoholic extraction, TLC and HPLC on C-18 column. The purplish red color changes along with pH variation and is relative stable at high temperature and under natural light. The acid-hydrolysate of the pigment contains cyanidin and glucoside. Maximum absorption was observed at wavelength 507 and 289nm for 40% cyanidin in acidified methanol. By means of IR spectra and G.C.-M.S. analysis, the structure of the cyanidin is probably similar to the structure of primuletin. This natural colorant is potentially useful in the food industry. |
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| Keywords: | Radish pigment HPLC Structure analysis |
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