| 半定量RT-PCR检测运动发酵单胞菌中外源基因转录水平的研究 |
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| 引用本文： | 马媛媛,邹少兰,张 鲲,洪解放,井 欣,刘 成,张敏华. 半定量RT-PCR检测运动发酵单胞菌中外源基因转录水平的研究[J]. 微生物学通报, 2009, 36(6): 0831-0836 |
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| 作者姓名： | 马媛媛 邹少兰 张 鲲 洪解放 井 欣 刘 成 张敏华 |
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| 作者单位： | 1. 天津大学石油化工技术开发中心,天津,300072;天津大学绿色合成与转化教育部重点实验室,天津,300072
2. 天津大学石油化工技术开发中心,天津,300072;天津大学绿色合成与转化教育部重点实验室,天津,300072;天津大学内燃机燃烧学国家重点实验室,天津,300072 |
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| 基金项目： | 国家自然科学基金项目资助(No. 30600369) |
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| 摘 要： | 本研究运用半定量RT-PCR法检测运动发酵单胞菌重组菌中外源基因xylB的转录水平。提取野生型运动发酵单胞菌CP4及其2个重组菌的总RNA, 检测无DNA污染后定量至同一浓度、并反转录为cDNA。观测目的基因xylB和内标基因16S rRNA的PCR扩增曲线、并确定合适的循环数, 选用相同量的cDNA为模板, PCR检测各样本中xylB相对16S rRNA的转录水平。结果表明野生型菌株CP4中xylB基因没有转录, 而两株重组菌中皆有xylB的转录本, 且转录丰度基本一致, 酶活测定也进一步证实该基因在重组菌中有效表达。该方法可用于鉴定运动发酵单胞菌中特定基因的转录水平, 是一种快速有效的检测方法。
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| 关 键 词： | 运动发酵单胞菌 半定量RT-PCR 基因表达 |
Research on Exogenous Gene mRNA Levels in Zymomonas mobilis by Semi-quantitative RT-PCR |
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| MA Yuan-Yuan,ZOU Shao-Lan,ZHANG Kun,HONG Jie-Fang,JING Xin,LIU Cheng and ZHANG Min-Hua. Research on Exogenous Gene mRNA Levels in Zymomonas mobilis by Semi-quantitative RT-PCR[J]. Microbiology China, 2009, 36(6): 0831-0836 |
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| Authors: | MA Yuan-Yuan ZOU Shao-Lan ZHANG Kun HONG Jie-Fang JING Xin LIU Cheng ZHANG Min-Hua |
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| Affiliation: | (1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China);(1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China);(1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China);(1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China);(1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China);(1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China);(1. Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China) (2. Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China)(3. State Key Laboratory of Eng |
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| Abstract: | |
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| Keywords: | Zymomonas mobilis Semi-quantitative RT-PCR Gene expression |
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