Transformation of arachidonic acid to 19-hydroxy- and 20-hydroxy-eicosatetraenoic acids using Candida bombicola |
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Authors: | Asmita Prabhune Simon R Fox Colin Ratledge |
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Institution: | (1) Department of Biological Sciences, University of Hull, HU6 7RX, UK;(2) Present address: Division of Biological Sciences, National Chemical Laboratory, Pune, 411 008, India;(3) Present address: Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA |
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Abstract: | Candida bombicola (ATCC 22214) and C. apicola (ATCC 96134), grown on glucose (100 g l–1) and arachidonic acid (5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid; AA), 1.25 g l–1, synthesized sophorolipid up to 0.93 g l–1. Acid hydrolysis of sophorolipid yielded 19-hydroxy-5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid (19-HETE) and 20-hydroxy-5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid (20-HETE) which were identified by TLC and GC-MS; the ratio of synthesis was 73:27, respectively. Conversion of AA by immobilized Candida bombicola, suspended in beads of 2% (w/v) calcium alginate for 96 h, gave an 83% conversion of 1 g AA l–1 to 19- and 20-HETE. There was no significant loss in the efficiency of the immobilized cells after ten uses. |
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Keywords: | arachidonic acid Candida bombicola eicosanoids hydroxyarachidonic acid sophorolipid |
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