18-Hydroxy-11-deoxycortisol: a new steroid isolated from incubations of the adrenal with 11-deoxycortisol |
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| Authors: | C E Gomez-Sanchez M F Foecking C H Shackleton M R Chavarri E P Gomez-Sanchez |
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| Institution: | 1. Section on Endocrinology & Genetics (SEGEN), Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD), National Institutes of Health (NIH), Bethesda, MD, USA;;2. Institute of Metabolism and System Research (IMSR), University of Birmingham, Birmingham, United Kingdom;;3. Division of Endocrinology and Diabetology, Department of Internal Medicine, University Hospital of Wuerzburg, Würzburg, Germany;;4. Institute of Pharmacology and Toxicology, University of Würzburg, Würzburg, Germany;;5. Calebiro D, Bathon K, Weigand I. Mechanisms of aberrant PKA activation by Cɑ subunit mutations. Horm Metab Res 2017; 1:CAS:528:DC%2BC28Xhsl2hsrfJ2781305449:307-314.Calebiro D, Bathon K, Weigand I. Mechanisms of aberrant PKA activation by Cɑ subunit mutations. Horm Metab Res 2017; 1:CAS:528:DC%2BC28Xhsl2hsrfJ2781305449:307-314.Calebiro D, Bathon K, Weigand I. Mechanisms of aberrant PKA activation by Cɑ subunit mutations. Horm Metab Res 2017; 1:CAS:528:DC%2BC28Xhsl2hsrfJ2781305449:307-314. Centre of Membrane Proteins and Receptors (COMPARE), Universities of Nottingham and Birmingham, Birmingham, United Kingdom; |
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| Abstract: | Cortisol has been shown to be metabolized in the zona glomerulosa of the adrenal gland through the same pathway involving the cytochrome P-450, corticosterone methyl oxidase by which corticosterone is transformed to 18-hydroxycorticosterone and aldosterone. When cortisol is the precursor, 18-hydroxycortisol and 18-oxocortisol are formed. 18-Hydroxycortisol can also be made at a similar rate in the bovine zona fasciculata and reticularis as in the zona glomerulosa. We studied the possibility that the formation of 18-hydroxycortisol in the zona fasciculata and reticularis might be through a different pathway involving initial 18-hydroxylation of 11-deoxycortisol before 11 beta-hydroxylation. Rat adrenal capsules or cores were incubated with 10 micrograms of cortisol or 11-deoxycortisol and the formation of 18-hydroxycortisol was measured by radioimmunoassay. Both capsules and cores transformed 11-deoxycortisol to 18-hydroxycortisol, but cortisol was only transformed in the capsular portion. Sixty-two rat adrenals were incubated with 10 mg of 11-deoxycortisol and the putative steroid, 18-hydroxy-11-deoxycortisol, was purified by TLC and HPLC and subjected to gas chromatography mass spectrometry. The mass spectra indicated that the steroid isolated was indeed 18-hydroxy-11-deoxycortisol. The function of this steroid is still unknown. |
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