转基因何首乌毛状根对8种活性成分的生物转化研究

目的：利用转基因何首乌毛状根悬浮培养体系对青蒿素等8种活性化合物进行生物转化研究。方法：分别向预培养9 d的何首乌毛状根培养体系中加入底物，共培养7 d后终止转化，通过TLC和/或HPLC检测转化产物，使用柱色谱法分离纯化产物。结果：化合物呋喃橐吾酮（6）发生了生物转化，并分离得到两个转化产物；对苯二酚（7）被转化，分离并鉴定出1个转化产物；青蒿素（8）发生了转化，分离得到两个转化产物。结论：转基因何首乌毛状根悬浮体系中存在多种生物酶系统，可对外源底物进行糖基化、氧化、还原和羟基化修饰。

Biotransformation of Eight Active Constituents by hairy Roots of Polygonum multiflorum Thunb

Objective: To investigate the biotransformation of eight active constituents chemicals by hairy roots of Polygonum multiflorum Thunb. Methods: Active compounds were added to the culture system of hairy roots of P. multiflorum after precultured for 9 d, then they were co-cultured for another 7 d. The products were isolated and purified by column chromatography. Results: Three of the eight active constituents were identified to be biotransformed, which were furannoligularenone (6), 1,4-benzenediol (7) and artemisinin (8). HPLC analysis of the biotransformation of 6 showed two new peaks except the peak of substrate, then the corresponding two compounds were obtained; Substrate 7 was converted into arbutin. In the case of 8, there were two new peaks and the corresponding two monomers have been also isolated. Conclusion: The result showed that there were several enzymes in the culture system of P. multiflorum hairy roots, which could processed glycosylation, oxidation, reduction and hydroxylation reactions.