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High throughput isolation of DNA and RNA in 96-well format using a paint shaker
Authors:Scott D Michaels  Richard M Amasino
Institution:(1) Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, 53706-1544 Madison, Wisconsin
Abstract:Most steps in plant nucleic acid isolation are easily adapted to 96-well format; however, tissue disruption typically is performed on samples individually and often is the rate-limiting step in sample processing. We have found that DNA and RNA isolation from Arabidopsis tissue can be carried out in 96-well format using a paint shaker fitted with an adapter for tissue disruption.
Keywords:96-well format  DNA isolation  high throughput  paint shaker  RNA isolation
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