High throughput isolation of DNA and RNA in 96-well format using a paint shaker |
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Authors: | Scott D Michaels Richard M Amasino |
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Institution: | (1) Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, 53706-1544 Madison, Wisconsin |
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Abstract: | Most steps in plant nucleic acid isolation are easily adapted to 96-well format; however, tissue disruption typically is performed
on samples individually and often is the rate-limiting step in sample processing. We have found that DNA and RNA isolation
from Arabidopsis tissue can be carried out in 96-well format using a paint shaker fitted with an adapter for tissue disruption. |
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Keywords: | 96-well format DNA isolation high throughput paint shaker RNA isolation |
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