The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis |
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Authors: | Sharipova Margarita Balaban Nelly Kayumov Airat Kirillova Yuliya Mardanova Ayslu Gabdrakhmanova Leila Leshchinskaya Inna Rudenskaya Galina Akimkina Tatiana Safina Dina Demidyuk Ilya Kostrov Sergey |
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Affiliation: | Department of Microbiology, Kazan State University, Kazan, Russia. Margarita.Sharipova@ksu.ru |
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Abstract: | The gene encoding for Bacillus intermedius serine proteinase was cloned and the complete nucleotide sequence was determined. Gene expression was explored in the protease-deficient strain Bacillus subtilis AJ73 during different stages of growth. Catabolite repression involved in control of proteinase expression during transition state and onset of sporulation was not efficient at the late stationary phase. Salt stress leads to induction of serine proteinase production during B. subtilis AJ73(pCS9) post-exponential growth. Expression of proteinase in B. subtilis deg-mutants may be controlled by DegU regulator. B. subtilis spo0-mutants failed to accomplish B. intermedius proteinase production. These data suggest complex network regulation of B. intermedius serine proteinase expression, including the action of spo0, degU, catabolite repression and demonstrate changes in control of enzyme biosynthesis at different stages of growth. |
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