On the role of the bacteriophage lambda int gene product in site specific recombination |
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| Authors: | H A Nash L W Enquist R A Weisberg |
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| Affiliation: | Laboratory of Neurochemistry National Institute of Mental Health National Institutes of Health Bethesda, Md 20014, U.S.A.;Laboratory of Molecular Genetics National Institute of Child Health and Human Development National Institutes of Health Bethesda, Md 20014, U.S.A. |
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| Abstract: | Integrative recombination of phage λ DNA occurs in extracts made from cells synthesizing int protein. In this paper we show that extracts of cells containing temperature-sensitive int protein are inactivated more rapidly by incubation at 38 °C than are wild-type extracts. This indicates that the int protein is directly involved in the recombination reaction. |
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