Benzo[a]pyrene-7,8-dihydrodiol: selective binding to single stranded DNA and inactivation of 0X174 DNA infectivity. |
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Authors: | W T Hsu D Sagher E J Lin R G Harvey P P Fu S B Weiss |
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Affiliation: | 1. The Franklin McLean Memorial Research Institute, The University of Chicago, Chicago, Illinois 60637 USA;2. The Department of Biochemistry and Microbiology The University of Chicago, Chicago, Illinois 60637 USA;3. The Ben May Laboratory for Cancer Research, The University of Chicago, Chicago, Illinois 60637 USA |
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Abstract: | When single-stranded ØX174 DNA is exposed to certain dihydrodiol derivatives of benzo[a]pyrene and benz[a]anthracene, inhibition of viral DNA infectivity is observed. Binding studies with labeled -7,8-dihydrodiol of benzo[a]pyrene and -benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide indicate that the diol preferentially reacts with single-stranded DNA, whereas the diolepoxide reacts equally well with both single- and double-stranded DNA, as well as with RNA. Also, the diol and diolepoxide derivatives show a marked difference in their capacity to complex with specific deoxyhomopolymers, i.e., Poly dI. These observations suggest that the diol and diolepoxide derivatives recognize different binding sites in nucleic acids, and that the diol derivative may play an important role in mutagenesis and carcinogenesis induced by polycyclic aromatic hydrocarbons. |
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