Regulatory mechanism of delayed-type hypersensitivity in mice. II. Effect of suppressor cells on the development of memory cells for delayed-type hypersensitivity |
| |
Authors: | S Tamura A Kojima Y Egashira |
| |
Institution: | 1. Division of Tumor Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 USA;2. The Departments of Microbiology & Immunology, and of Pathology, University of Washington, Seattle, Washington 98104 USA |
| |
Abstract: | Murine lymph node cells (LNC), which we showed previously to noncompetitively inhibit antibody-dependent cellular cytotoxicity (ADCC) to an erythrocyte target, were tested for their ability to inhibit ADCC to a tumor target, EL-4. Both a 4-hr 51Cr-release cytotoxicity assay and an overnight 125IUdR (iododeoxyuridine) postlabeling cytostasis assay were used. Normal autologous lymph node cells inhibited spleen cell-mediated ADCC in both assays. Inhibition by LNC was dose dependent, but comparable numbers of sheep erythrocytes did not inhibit, indicating that LNC-mediated inhibition was not simply a matter of crowding. Inhibitory activity was enriched in LNC after removal of Fc receptor-bearing cells on EA monolayers. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|