DNA methylation,retroviruses, and embryogenesis |
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Authors: | Rudolf Jaenisch Klaus Harbers Detlev Jhner Colin Stewart Heidi Stuhlmann |
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Institution: | Rudolf Jaenisch,Klaus Harbers,Detlev Jähner,Colin Stewart,Heidi Stuhlmann |
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Abstract: | By exposing preimplantation embryos to Moloney leukemia virus (M-MuLV), we have previously derived substrains of mice designated as Mov-1-Mov-13 which genetically transmit the virus from one generation to the next. In some of the substrains the inserted viral genome becomes activated at specific stages of embryogenesis and the available evidence suggests that these viral genomes are developmentally regulated. To investigate the effect of cellular differentiation on virus expression, M-MuLV was introduced either into preimplantation or postimplantation mouse embryos or into embryonal carcinoma (EC) cells. Whereas preimplantation embryos or EC cells are not permissive for virus expression, efficient replication occurred in postimplantation embryos or in differentiated cell lines. The viral genomes introduced into early embryonal cells were highly methylated and noninfcctious when analyzed in the adult. In contrast, viral genomes introduced into postimplantation embryos or into differentiated cells remained unmethylated and were infectious in a transfection assay. These results demonstrate an efficient de novo methylation activity which appears to be involved in repression of genes introduced into pluripotent embryonal cells and which is not observed in cells of the postimplantation embryo or in differentiated cells in tissue culture. |
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Keywords: | de novo methylation of retroviral genomes virus expression during embryogenesis embryonal carcinoma cells maintenance methylation preimplantation mouse embryos postimplantation mouse embryos infectivity of retroviral genomes integration and methylation of retroviral genomes |
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