A novel highly boron tolerant bacterium, <Emphasis Type="Italic">Bacillus boroniphilus</Emphasis> sp. nov., isolated from soil,that requires boron for its growth |
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Authors: | Iftikhar Ahmed Akira Yokota Toru Fujiwara |
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Institution: | (1) Biotechnology Research Centre, University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan;(2) National Agricultural Research Centre, Park Road, Islamabad, 44000, Pakistan;(3) Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan;(4) SORST, JST, Chiyoda-ku, Tokyo, Japan |
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Abstract: | Three strains of gram-positive, motile, rod-shaped and boron (B)-tolerant bacterium were isolated from naturally B containing
soil of Hisarcik area in the Kutahya Province, Turkey. The strains, designated as T-14A, T-15ZT and T-17s, produced spherical or ellipsoidal endospores in a terminal bulging sporangium. The strains required B for the
growth and can tolerate more than 450 mM B. These also tolerated up to 7.0% (w/v) NaCl in the presence of 50 mM B in agar
medium but grew optimally without NaCl. The temperature range for growth was 16–37°C (optimal of 30°C), whereas the pH range
was 6.5–9.0 (optimal of 7.5–8.5). The DNA G + C content was 41.1–42.2 mol% and the predominant cellular fatty acid was iso-C15:0. The major respiratory quinone system was detected as MK-7 and the diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis of 16S rRNA gene sequences
data and DNA–DNA re-association values, we concluded that the three strains belong to a novel species of the genus Bacillus, the type strain of which is T-15ZT and for which we proposed the name, B. boroniphilus sp. nov. (DSM 17376T = IAM 15287T = ATCC BAA-1204T). |
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Keywords: | Bacillus boroniphilus Boron tolerance Boron requirement Boron contaminated soil Hisarcik area Taxonomy |
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